Into the chromosome 4, all around three QTLs was basically during the 66
Package plots appearing the consequences of your own marker genotypic classes (AAAA, AAAB, AABB, ABBB, BBBB) of cuatro candidate SNPs towards tuber sugar attention (GLU); bud-stop fry color (FBE); stem-tuber fry colour (FSE); ID, Idaho
The new co-localization regarding multiple QTLs in the readiness locus part inside analysis is actually similar to the noticed designs off phenotypic correlations. 01) with several qualities overlapping an identical hereditary condition (Fig. 1). Such as, EBID11 and you can VWID11 ratings both demonstrated a robust negative correlation which have MAT95ID and you can MAT120ID. It trend can get give an explanation for biggest impression QTL which have later maturity closely on the put off start of VW and you will EB, or alternatively, very early readiness causing very early onset of VW and you will EB. A relatively large correlation has also been seen between vine maturity and you can growth habit, which have early readiness of this much more distribute progress behavior. In contrast, the lowest correlation (roentgen ? 0.2) between vine readiness and you can each other tuber sugar amount and you may bud-end fry color try in keeping with the latest models of for the QTL effects.
Tuber glucose, bud-prevent and you will stalk-prevent fry color
Along with chromosome 5, high QTLs for sugar, bud-stop and you can base-stop fry colour was indeed thought of from inside the overlapping aspects of chromosomes cuatro, 6, 10, and you may eleven. 0 cM into the LOD ratings between 4.5 to 6.0 (Table step 3, Fig. 4). This type of QTLs told me between eight.2 and you can eleven.3% of the feature difference. Investigation of various easy patterns expressed an individual top-fitting design for everyone about three faculties, that have a double-simplex allele (AAAB ? AAAB) to your homologous chromosomes H2 and you can H5 out of RGR and you can Pr, respectively (Desk step three, Additional document 8: Shape S4). To have sugar, the brand new B allele try from the high sugar focus, while you are for bud-stop and you will stem-end fry colour, the current presence of new B allele shown down reflectance beliefs (Fig. 3b).
LOD get delivery away from QTLs for a few qualities along the a dozen potato chromosomes (Chr). Abbreviations is actually as in Fig. step 1
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, https://datingranking.net/es/tatuaje-de-citas/ significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
eight Mb) which has several family genes of the starch/sugar inter-transformation together with hexokinase (HXK-six, PGSC0003DMG400016521), fructokinase (FRK-6/1, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and you may invertase (INV-na-6/1, PGSC0003DMG400026107, INV-6/2, PGSC0003DMG400033142) genes.